Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The effects of vincristine on WRL68 cells and cell viability were assessed using the CCK-8 method. Cells were treated with different concentrations of vincristine (0.00, 0.01, 0.10, 1.00, 5.00, and 10.00 μg/mL) for 48 h at 37 °C. Data are presented as the means ± SEM determined from five independent experiments. **** P < 0.0001compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: CCK-8 Assay, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The averaged absorption spectra of WRL68 cells that were untreated (control group, black) and treated with 0.120 μg/mL vincristine (treated group, red).

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The vector-normalized averaged second-derivative spectra of the WRL68 cells that were untreated (control, black) and treated with vincristine (red).

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from the WRL68 cells in the lipid region (3000–2800 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.925, R 2 Y = 0.704, Q 2 = 0.651). The x - and y -axes indicate the first component t1 and the first orthogonal component to1. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean ν as (CH 2 )/ν as (CH 3 ) and ν s (CH 2 )/ν s (CH 3 ) peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated cells, **** P < 0.0001compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from WRL68 cells in the protein region (1800–1480 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.961, R 2 Y = 0.617, Q 2 = 0. 511). The x - and y -axes indicate the first component t1 and the first orthogonal component to1, respectively. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean protein amide I/amide II (1670–1646/1563–1531) and α-helix/β-sheet (1670–1646/1644–1621) absorption band peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated cells. **** P < 0.0001 and *** P < 0.001 compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from the WRL68 cells in the fingerprint region (1480–900 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.597, R 2 Y = 0.873, Q 2 = 0. 754). The x - and y -axes indicate the first component t1 and the first orthogonal component to1, respectively. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean nucleic acid ν as (PO 2 – )/lipid ester ν(C=O) (1269–1201/1754–1723), nucleic acid ν as (PO 2 – )/protein amide II (1269–1201/1563–1531), nucleic acid ν s (PO 2 – )/lipid ester ν(C=O) (1105–1070/1754–1723), nucleic acid ν s (PO 2 – )/protein amide II (1105–1070/1563–1531), and nucleic acid ν s (dianionic phosphate monoester)/lipid ester ν(C=O) (984–948/1754–1723) peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated groups. **** P < 0.0001 compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: Assignments of the Most Relevant IR Absorption Bands of WRL68 Cells, Together with the Related Vibrational mode 28 − 30

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques:

Journal: Translational Cancer Research

Article Title: Analysis of the role of POC1A in the development and progression of hepatocellular carcinoma

doi: 10.21037/tcr-23-2398

Figure Lengend Snippet: Analysis of the expression of POC1A in various classifications of HCC patients, as well as in HCC and liver cancer cell lines. (A) Comparison of POC1A between cancer tissue samples and normal tissue samples. (B) Comparison of the expression of POC1A in different genders. (C) Comparison of the expression of POC1A in different age groups. (D) The expression levels of POC1A were all higher in the Caucasian, African-American, and Asian HCC patients than the normal patients. The expression of POC1A was significantly higher in LIHC tissue samples from Asian patients than LIHC tissue samples from Caucasian patients. (E) The expression of POC1A in individual cancer stages. (F) The expression of POC1A in different transistor grades. (G) The expression of POC1A in tumor (T) stage. (H) The expression of POC1A in node (N) stage. (I) The expression of POC1A in metastasis (M) stage. (J) Analysis of POC1A expression in normal liver tissue and cancer tissue samples from patients with hepatocellular carcinoma using immunohistochemical methods in the Human Protein Atlas database. Liver sample: https://www.proteinatlas.org/ENSG00000164087-POC1A/tissue/liver#img , hepatocellular samples: https://www.proteinatlas.org/ENSG00000164087-POC1A/pathology/liver+cancer#img . Scale bar: 100 µm. (K) Analysis of POC1A mRNA expression in LO2 and HepG2 cell lines by qPCR assay. *, P<0.05; **, P<0.01; -: no significance. TCGA, The Cancer Genome Atlas; HCC, hepatocellular carcinoma; LIHC, liver hepatocellular carcinoma; mRNA, messenger RNA; qPCR, quantitative polymerase chain reaction; POC1A , POC1 centriolar protein A.

Article Snippet: The human normal hepatocyte cell line LO2 (Servicebio Technology Co., Ltd., Wuhan, China) was cultured in Roswell Park Memorial Institute 1640 medium, and the hepatoma cell line HepG2 (Procell Life Science & Technology Co., Ltd., Wuhan, China) was cultured in Dulbecco’s Modified Eagle Medium (Hyclone, Thermo Fisher Scientific Inc., Waltham, MA), supplemented with 10% fetal bovine serum and antibiotics (10,000 U/mL penicillin and 10 mg/mL streptomycin) (Procell Life Science & Technology Co., Ltd.).

Techniques: Expressing, Comparison, Immunohistochemical staining, Real-time Polymerase Chain Reaction